FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4739760
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATACCACCCCACCACACTCCTACCAATAACGGTAACTATTCTATGTTT48800.10295879960166761No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGCGG44350.038.59016856
GGGGAAA89600.038.20254570
GGGTGGC40300.037.4311954
GGTGGCG35950.037.28733455
AAGATCG151250.037.1442386
GCGGCGT14450.035.84720259
CGTGGGC19850.035.79290463
GGGTGGA54650.035.73596268
CGGGGGT58200.035.54069551
TGGCGGG67950.035.12823557
GGCGTGA10600.034.9994767
TGGGCGT29700.034.99946665
GGGCGTG28850.034.9388166
TGGCGGC14950.034.64830457
ATCGGGG92950.034.6417549
GCGTGGA39050.034.23763368
GTGGGCG36800.034.14350564
TATCGGG118850.034.10128848
CCGGCGT22000.033.88584559
GTGGAAA98400.033.75456670
Produced by FastQC (version 0.11.5)