FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3432635
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAAAA205650.041.18575370
TGGAAAA124950.040.1953770
GAAGATC72300.038.538825
AAGATCG284300.038.045676
GCGTGAA161800.037.81144368
GGCGTGA85500.037.2098767
AAAGATC92500.037.0479855
TGCGTGA78800.036.99807467
CGCTTGC5550.036.5759323
GTGAAAA186450.036.30399770
GCCGTCG199700.036.20867558
TCGTCGG86050.036.1992462
GGGCGTG60350.036.1882666
GGGAAAA52300.036.07010770
CCGTCGT194000.036.06379759
TGGGCGT64400.035.65154665
GGGTGGA58350.035.6291968
CTGCGTG92250.035.5874566
GTGTGCC18700.035.5608754
GTGGGCG54200.035.5159864
Produced by FastQC (version 0.11.5)