FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3813748
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTGA37350.039.54448767
GCGTGAA57550.039.28721668
GGGGAAA79200.038.40242870
AAGATCG175800.037.290666
GTGGCGG23250.037.18245356
CGTGAAA61950.037.0053169
GCCGTCG80600.036.73663358
TGCCGGC30150.036.5668357
CGTCGGC42600.036.4785463
TGGCGGG40000.036.2246757
TGGAAAA91450.036.128770
TCGTCGG39150.036.11716562
CCGTCGT84050.035.8533359
GTCGGCG47700.035.58667464
CGCGGGT16200.035.43177451
TCGGCGT60100.035.4073365
CCGGCGT25150.035.0692659
CGTCGTC53350.035.03248260
TGCCGTC146300.034.76044557
GGGAAAA48400.034.71042670
Produced by FastQC (version 0.11.5)