FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5946776
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53150.027.98627755
CGAGTGG112000.026.59322740
TATCTCG82950.026.53956248
GCCGTCG24050.026.34043758
GTATGCC57950.026.21173754
GGGGGGA17900.026.20060368
ACGAGTG114150.026.09234639
CACGAGT114850.025.99426338
GTCGGCG12550.025.93574364
CTCGTAT61900.025.78301251
GTCACGA115500.025.7570736
TGCCGTC45300.025.7279757
GAGTGGA115950.025.71747641
AGTCACG116050.025.63499535
CGTCGGC12300.025.60925163
GTGGATA116300.025.45951743
TCTCGTA63550.025.27880950
ATCTCGT84050.025.23448249
CCGTCGT26500.025.22591859
CGTATGC60100.025.2158153
Produced by FastQC (version 0.11.5)