FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5572524
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT11700.028.1193351
TCGGGGG15100.027.35067250
ATCGGGG20000.025.724749
CGTACGT77750.025.7038841
GGGTGGC10950.025.25084754
GTACGTA80150.025.15254642
CCGTACG80150.024.93420840
TGGCGGG15300.024.70559557
CGTAATC68900.024.68766645
ACGTCTG82650.024.43407622
GAGATCG26350.024.3090826
CGTCTGA83950.024.05570423
ACGTAAT83400.023.71075444
GATCGGA84600.023.705618
ACACGTC85300.023.63395720
AATCGGG24100.023.52669548
TCCAGTC85150.023.51117532
AGATCGG85300.023.429017
CACACGT86500.023.42747119
GCACACG86750.023.23892218
Produced by FastQC (version 0.11.5)