FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4150577
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57040.1374266758573567No Hit
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG50120.12075429512571384Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT64650.047.1767921
GGGGAAA95600.042.02841670
GGGAAAA46700.039.04629570
ACGAGTG4150.037.95101538
GGGTGGA45250.036.8942668
CGGAGTC4300.035.813647
GTGGAAA69000.035.3036170
GTGAGGT4500.034.9996942
CGAGTGA4650.033.8706739
GCGTGGA21800.033.55434868
GGTGGAA52050.032.27598669
GAGTCAC5200.031.6343369
CACTCTT170650.031.231091
GTCGCCT6550.030.99172212
ACGGAGT5200.030.9649966
GTCACTC5200.030.96089211
CACGAGT5250.030.66602937
GCGGGGA20400.030.02388468
CGGGGAA20250.029.90061269
TTGCGGG44150.029.33121157
Produced by FastQC (version 0.11.5)