FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210118
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54220.12878498892430093No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT49150.045.6652831
GCGTGAA64000.039.15559468
GAGTCAC5300.038.3016979
CGTGAAA67400.037.69966569
TGCGTGA30100.037.32495567
GGCGTGA37100.037.26352767
CTGCGTG32050.037.12886466
GGGTGGA34800.036.00514268
GGGGAAA38800.035.1798270
GCGTGGA50400.034.86052768
TCGGCGT60000.034.18275565
GTGAAAA85150.034.11569270
GTGGAAA89500.033.94356570
AGTGGCG5300.033.6786839
GTCGGCG42050.033.3763764
ACGAGTG5700.033.15733738
TCGTCGG41500.032.72234362
CGGAGTC6000.032.6665047
GGGCGTG29600.032.63458666
CGTCGGC44650.032.05993363
Produced by FastQC (version 0.11.5)