FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5582944
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTG7300.034.52013438
GTGAGGT8000.031.93711542
ACACTCT22500.031.1277431
CACGAGT8350.031.01759137
CGAGTGA8150.030.91959439
CGGAGTC8500.030.8819797
GAGTCAC8400.030.4163049
CGTATGT8400.030.4160358
GTCACTC8700.029.367211
GGAAAAA84450.028.80367970
GGGTGGA17950.028.6626166
ACGGAGT9650.027.5676446
GCACTTA9400.027.55285868
GGAGTCA9450.026.6663468
ACTCAGC9850.026.29386514
ACGTATG10450.026.12385457
TTGGCGG20300.025.51693354
GAGATCG60550.025.4361826
CAAACGG10500.025.0023863
GCGAGAT10800.024.9563084
Produced by FastQC (version 0.11.5)