FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3792391
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT17100.037.061011
GGGTGGA16200.034.9995966
CGAGTGA5200.034.32652339
ACGAGTG5200.033.65345438
GCGTGGA21450.033.28632466
CGGAGTC5850.032.3073167
CACGAGT5850.031.7090337
CGTGGTG15600.031.63466358
GAGTCAC5700.031.3154269
GCCGTGG16000.031.06254856
CCGTGGT16550.030.8761857
GTGAGGT5800.030.77550342
TCGGCGT15250.030.2947363
TGCCGTG23650.030.19032155
GTGGAAA43850.029.69178868
GCACTTA6250.029.67965368
GCCGTGT7000.029.50004456
GGAAAAA77800.029.33127870
CCGGGGT19650.029.21123957
CGTGGAA24750.028.84814867
Produced by FastQC (version 0.11.5)