FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_vk11-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_vk11-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5759292
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG83780.14546926948659664Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71900.12484173401869536No Hit
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC70020.12157744389414532Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT108700.050.638131
GGAAAAA166000.041.13500270
GGGAAAA246450.035.6883170
CGGAGTC6800.033.970147
CCGGGGT19050.032.88670757
ACGAGTG7250.031.86165238
AAAGATC98900.031.5344495
CACTCTT259300.031.1124781
TTGGCGG56050.030.9719454
GTGAGCG2750.030.54505263
TGCCGGG33000.030.54505255
GAGTCAC7450.030.536519
CGTGAGA11750.030.385483
GCGTGGA7950.030.37695966
GGTTGGC57850.030.3712552
CGTATGT7850.030.31807158
GTTGGCG52050.030.19172553
GCGAGAT30750.029.8235954
CGAGTGA7850.029.42636339
GTGAGAT28550.029.424494
Produced by FastQC (version 0.11.5)