FastQCFastQC Report
Tue 5 Jan 2021
HKVGTAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVGTAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11723269
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3227902.7534128919160685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT64150.032.1872671
GCGAGAT11650.028.1789474
AAGATCG124050.026.7173966
AAAGATC41800.026.318485
TCACGTG39950.025.96631637
GAGATCG86400.024.939986
GGAAAAA73750.024.82972170
CGCGAGA6200.024.789363
GCGAAGA6650.024.1624243
GGAGATC17650.023.546635
CACGTGG14150.023.53328338
CGCGAAG4100.023.002792
CACGTGA30800.022.87497738
GAAGATC37900.022.668485
GCGCTAG2800.022.4551052
GCCCACA10550.022.187871
TGAGATC25800.021.9291525
CCCTACA81950.021.8167519
CGAGATC39350.021.7444215
CACACGT309550.021.58487719
Produced by FastQC (version 0.11.5)