FastQCFastQC Report
Wed 25 Jan 2023
HKV2TDRX2_n02_br224.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKV2TDRX2_n02_br224.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5414870
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC1059511.9566674730879967No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG984071.8173474155427556No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC98500.18190649082988142No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG95670.17668014190553052No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA76190.14070513234851437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)