FastQCFastQC Report
Wed 25 Jan 2023
HKV2TDRX2_n01_br240.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKV2TDRX2_n01_br240.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3356831
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC567851.6916252262922977No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG565431.6844160459671638No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG54720.16301088735179103No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC49980.14889042671495825No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA44990.13402521604453724No Hit
GTCATAAACTCTTCTCTTCCATATGACTATCCCCTTCCCCATTTGGTCTA34950.10411605469563406No Hit
GTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCAC34880.10390752468622937No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)