FastQCFastQC Report
Wed 25 Jan 2023
HKV2TDRX2_n01_br236.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKV2TDRX2_n01_br236.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194997
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG384211.75038963606784No Hit
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC365351.6644669673808212No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG35850.16332596354345813No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC32620.1486106814724576No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA24180.11015960386278431No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)