FastQCFastQC Report
Wed 25 Jan 2023
HKV2TDRX2_n01_br228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKV2TDRX2_n01_br228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9980339
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC1265611.2681032177363916No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG1257531.2600073003532246No Hit
CGTCTAGACTGTGTGCTGTCCTTTCATGCCTTGACGGCTATGTTGATGAA165640.16596630635492443No Hit
GGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG125120.1253664830423095No Hit
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC113420.1136434343562879No Hit
GTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCAC110000.1102166970480662No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)