FastQCFastQC Report
Fri 1 Apr 2022
HKTVNAFX3_n01_10677spikein-in.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTVNAFX3_n01_10677spikein-in.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8050048
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGGGGGGTGG118450.14714197977453053TruSeq Adapter, Index 7 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGGGGGGGGG95930.11916699130241212TruSeq Adapter, Index 7 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGGGGGGTTG91010.11305522650299725TruSeq Adapter, Index 7 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTGG84240.10464533876071297TruSeq Adapter, Index 7 (97% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)