FastQCFastQC Report
Fri 1 Apr 2022
HKTVNAFX3_n01_10676-hop1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTVNAFX3_n01_10676-hop1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8295563
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT4348655.24213968358748TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTTT309930.3736093620167793TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGAGCTCGTAT289060.34845133476775475TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATGTCGTAT215920.2602837203454425TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGCTCTCGTAT153880.18549675290272644TruSeq Adapter, Index 19 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCGCGTAT114290.13777244534216665TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAA87150.10505616074520802TruSeq Adapter, Index 19 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)