FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7808654
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG268950.3444255565683919No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG97150.1244132471486123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTG34400.037.5259441
TGCGTCG23850.024.8565331
TGTCGGC26750.023.54896730
AGTCGTG42650.023.0293251
CGCGTAG4950.022.6527922
CGGCGGC29900.021.53637333
CGCCAAG17550.021.3869511
AAGCGTC27800.020.5676671
CGCGAAT9300.019.9910281
CGCGAAG9700.019.8899271
GTGGGAC32800.019.7389419
CGGCAAG20800.019.731711
CGGCAAA59200.019.1984231
CGCACAG6250.019.0827561
TCGCGTC2951.8189894E-1219.025681
ACGCGTC5000.018.9424421
CGGGCAG6800.018.5710221
GTGCGTC9500.018.4624181
CGCGGAG5900.018.4311281
CGGGAAG90200.018.3948751
Produced by FastQC (version 0.11.5)