FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683367
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG143410.3893448575718901No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG55000.14931990214388086No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC42410.11513921908949067No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC13850.032.09131630
TGTCGTG10900.029.2838611
CGGCGGC14950.028.79370133
GTGGGAC16750.026.32591219
TCGGCGG17600.024.65713532
CGCGCAA2600.022.9344881
CGGGAAG44900.022.4988141
GAATGTC19200.022.2378227
GTCGGCG19500.021.895731
GCGGCAG20750.021.75760535
CGCCAAG7800.021.58541
GGCGGCA20450.021.56278834
CGGCAAG8950.021.5552521
AATGTCG20850.020.98154828
CGGCAGT20800.020.69547536
CGCGAAA4850.020.250221
CGCGAAG6000.019.8765541
CGAGCAG6650.019.516121
AGCGGAG13200.019.3982431
TGAGACC22050.019.3632845
Produced by FastQC (version 0.11.5)