FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247834
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141810.43662945827896377No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC37290.11481498130754218No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA33410.10286855793738227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACC3800.047.0075763
TCGTGAC4300.041.5601542
TGTCGTG13950.037.969981
GTGACCT5050.036.0561644
AAAGGGG152250.035.3305270
CTAGATC22500.033.454665
CGCGTAG2350.032.8042562
GCAGATC24050.032.3225364
CGGCAAG6800.031.9830861
CGGCTAG1650.031.8892941
TCGCGTA2750.030.6137221
TGCGTCG9200.030.5028041
CGAGATC26850.029.860145
TTCGTGA7300.029.7924671
AAGGGGG91050.029.02020570
CGGGCAG2700.028.5822581
CATTAAA216150.028.33411261
ACGCGTC1750.028.062581
CGTGCAG2400.027.7702621
TCATTAA215750.027.6891460
Produced by FastQC (version 0.11.5)