FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12359200
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG396130.320514272768464No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGC42500.025.03312733
TGTCGGC42150.024.99190330
TGTCGTG35900.024.8167131
CGCGAAG15200.024.691371
TTGCAGG45350.023.2032571
CGCGAAA17150.023.111031
CGGCAAG24850.023.0073281
TCGGCGG48850.021.77907632
TGCGTCG17250.020.9436861
AAAGGGG123150.020.74525870
CAGATCG57300.020.652715
AAAAGGG239950.020.62334470
GTCGGCG52650.020.2736531
CGAGCAG19750.020.2461641
CGCGGAG8700.020.1583841
CGCCAAG24200.020.0017831
CGGGAAG103250.019.8393651
CGGGCAG9450.019.6720241
GGCGGCA57150.018.73845934
GAATGTC57600.018.47047427
Produced by FastQC (version 0.11.5)