FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6778516
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG267480.39459964393386393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG10100.029.1758671
CCGGATC42950.027.54082555
CGCCGGA48800.027.32301153
TCGCCGG67100.027.27737652
GTCGCCG147000.027.2114951
GGTCGCC147850.027.1260650
CGGATCA44850.026.4521356
ATCTCGG101050.026.00920342
GCCGGAT65800.026.00796754
CGGCAAG20000.025.9595761
CGCCGTA66950.025.8748653
TCGCCGT83900.025.86146752
GATCTCG102150.025.66136241
CTAGATC36950.025.4888535
AAGATCG183150.025.1356246
CCGTATC68350.025.08886355
GCCGTAT83900.024.90209254
CAGATCG80200.024.7526195
TGGTCGC116100.024.74767149
CGCCGGG62000.024.72326953
Produced by FastQC (version 0.11.5)