FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6835148
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240680.3521211245169819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG179750.036.5843370
CGGCAAG19100.030.115221
CGCGCAG2500.029.4614981
CCGTATC283100.028.81576255
CGTATCA287250.028.53346656
AAAAGGG365450.028.32744670
CATTAAA334050.028.18281661
CGCCGTA311050.028.17291653
GTATCAT275900.027.8553357
TCATTAA331900.027.74323860
TCGCCGT315500.027.69810552
CCGGATC55400.027.41622555
CTAGATC40000.027.3949785
GCCGTAT337850.027.38830854
GTCGCCG391900.027.3707151
CGAGATC40950.027.015925
CGCCGGA71750.026.58289753
GGTCGCC433500.026.40719850
ATCATTA343250.026.31549359
ATCTCGG349000.026.25257542
Produced by FastQC (version 0.11.5)