FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3539202
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129300.3653365928251623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG80550.035.53986470
AAAAGGG173650.029.80721370
CGTATCA146600.029.3155856
CCGTATC146100.029.15241255
CGAGATC20700.028.7539045
CGCCGTA165300.028.22223553
CGGCAAG7600.028.1551531
TCGCCGT167500.028.03960252
CATTAAA171550.027.58164261
GCCGTAT175500.027.4593954
GTCGCCG200550.027.43202451
GTATCAT144850.027.42278157
TCATTAA169700.027.40799560
GGTCGCC220650.026.61435950
CTCGGTG171350.026.46969244
TGGTCGC211250.026.39046549
GATCTCG172300.026.20150841
TCTCGGT169950.026.19352343
ATCTCGG177200.025.9908342
CCGGATC22800.025.94095655
Produced by FastQC (version 0.11.5)