FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402048
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG248990.3889224198256558No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG205850.039.15323670
AAAAGGG424750.032.16629870
CGAGATC48350.032.0066645
CGCGCAG3300.031.888231
GCAGATC46150.029.7463874
CCGTATC364500.029.65821555
CGTATCA367400.029.57651956
CATTAAA422100.029.25149361
TCATTAA416700.029.10121760
CGCCGTA409150.028.64532953
CTAGATC44200.028.516415
CCGGATC62400.028.49074455
CAGATCG103250.028.4841885
AAGATCG234400.028.466176
GTATCAT357100.028.4011957
CGCGGCC2100.028.3307266
TCGCCGT413350.028.23595852
GCCGTAT434500.028.23055854
CGGATCA63800.028.08496556
GTCGCCG500050.028.04338851
Produced by FastQC (version 0.11.5)