FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4833825
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138400.28631570236820736No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG91710.18972552792043568No Hit
CGGCAAAATGTTAGTGCAGGAAAGCGGGAAGGAATAAGAAGCAACTAAAC50670.10482381964593257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGGC15200.022.3335633
TGTCGGC14950.022.00475330
GTCGGCG16700.020.1179931
CCCGGGT4250.019.76255846
CGGGCAG5700.019.6985451
CGGCAAG14700.019.5729181
CGGGAAG65950.019.313051
CGCGGAG3650.019.226321
CCCCGAC3001.8189894E-1218.66463922
CGGCAAA44250.018.6343251
TCGGCGG18350.018.49973732
CGCGAAT7300.018.2650051
GTGGGAC18700.018.15310919
CGCGAAG6850.017.9281921
CGGGGAG6900.017.7982791
CTAAACG45600.017.72833621
GTGCAGG27500.017.68898814
TGCGGTG9750.017.6339871
CGCACAG3400.017.5440181
CGCGAAA9450.017.4511931
Produced by FastQC (version 0.11.5)