FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056092
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG287170.40698165500109695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG263600.040.3745570
CGAGATC55100.034.373275
AAAAGGG536200.032.04076470
CTAGATC56250.031.9278745
CGCGAGA8350.031.0425623
GCAGATC56100.030.4596234
CAGATCG128350.029.8944025
CATTAAA534300.029.55429561
CCGTATC468700.029.51611955
CGTATCA471000.029.51316356
CCGGATC83850.029.5082755
TCATTAA528200.029.27940460
CGGATCA85850.029.02465856
CAAGATC92600.028.9596255
AAGATCG288150.028.9134336
CGCCGTA517700.028.90593153
TCGCCGT522100.028.66232352
GTCGCCG631750.028.50702751
CGCCGGA105650.028.42138353
GTATCAT456600.028.4204757
Produced by FastQC (version 0.11.5)