FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3872219
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150020.38742643430033274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC10700.027.14766530
CGCGAAG5450.025.7531571
CGGCGGC11700.024.82702833
CGCGAAA6350.022.6556841
TCGGCGG13300.022.10345632
TGTCGTG13500.021.573041
CGGCCAG2301.2732926E-1121.3583281
CGGCAAG8900.021.289761
GTCGGCG13700.021.2029231
CGGCCTA1751.2340752E-720.0506741
CGCGCAG1408.080877E-620.0506731
CGGGAAG34200.019.0833171
CGCGGTT1852.2156564E-718.9668541
AGTCCGC17600.018.69141212
GAAGCGT17650.018.63846269
GAATGTC15800.018.60631427
CGGGCAT2851.2732926E-1118.4677281
GGGTCTC17850.018.42986761
GTCCGCA18100.018.3681913
AAAGGGG37100.018.30015470
Produced by FastQC (version 0.11.5)