FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945761
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99560.33797718144818945No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG2050.029.0941431
CCGACCC500.0072156327.99725256
CGGGCTG1251.0761323E-725.2605611
TCGCGTC851.2885302E-424.7652531
TGTCGGC9500.023.57703230
CGGGCTA1202.1260294E-623.389411
CGGCGGC9900.022.62442433
CGAGCAG5150.021.7998351
CGCGCAG850.00373978420.637711
CGGGCAG2504.0017767E-1119.6471041
TGCGGTA6300.018.9342821
CGCGAAA4700.018.661761
GTGGGAC12250.018.56960519
GTCCGCA13250.018.48906513
CGGGAAG27000.018.3217031
AGTCCGC13400.018.28209512
GGGTCTC13400.018.28209561
TCGGCGG12300.018.20990432
GTCGGCG12350.018.13617731
CGGCAAG6150.017.6846751
Produced by FastQC (version 0.11.5)