FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2788513
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG118600.4253162886455972No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA41270.14800002725466943No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC33850.1213908631589668No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG32250.11565303801703633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC11900.028.23248930
CGGCGGC12500.027.15729933
GTGGGAC14450.023.00809519
CGCACAG2600.022.9395221
CGCGGAT1553.425339E-822.634821
TCGGCGG15200.022.33330532
CGGGAAG31750.022.2106421
GCAACCC5550.022.07027438
GAATGTC15600.021.76065827
GTCGGCG15650.021.46751431
CGAGCAG5200.020.9154471
AATGTCG16400.020.69916228
TGAAGCG17600.020.67976268
CGGCCTG850.00373981820.6376321
CGGCAAG7100.020.259761
CCCCCGT1055.472909E-419.99801319
TGTCGTG8600.019.9897081
GGCGGCA17150.019.7939534
GAAGCGT18650.019.70313569
CGGCTAG1257.688571E-519.6470241
Produced by FastQC (version 0.11.5)