FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2962709
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82630.2789001552295551No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA52960.17875532156549967No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC52690.17784399345328888No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG47370.15988745435343127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGGC16600.028.4616530
CGGCGGC17400.027.15307233
GTGGGAC17650.026.9658419
AATGTCG20000.023.79815728
GAATGTC19750.023.74499727
CGGCAAG7600.023.5390621
TCGGCGG19950.023.50695232
GTCGGCG20500.023.04699531
CGCGGAG2300.022.8768371
GAAGCGT24150.022.1714569
CGCGAAG4300.022.0256061
AAGCGTA24650.021.5797570
CGACCCG1154.1247913E-521.30269827
CGGGAAG41650.021.223551
TGAAGCG25700.020.69808868
CAGAATG22850.020.67638625
CCGTCAC5600.020.6227166
GGCGGCA23000.020.5418934
GCGGCAG23300.020.42760335
TGCGGTG5200.020.2372021
Produced by FastQC (version 0.11.5)