FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592344
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72170.2783966942658845No Hit
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG26780.10330419111043905Illumina Single End PCR Primer 1 (100% over 32bp)
GGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCT26480.10214693728918692Illumina Single End PCR Primer 1 (100% over 39bp)
AGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATC26270.10133685961431045Illumina Single End PCR Primer 1 (100% over 38bp)
GGGAAAGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG26060.10052678193943397Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAG503.4374334E-642.0797921
CGCGGAG1600.037.2581441
TGTCGTG9850.031.6844431
CGCGAAG3100.029.4106041
TGCGTCG5450.025.0934521
CGCGCTG700.001213470125.0474931
ACGCGTC1551.2641976E-924.8858971
CGGGCTG1102.8827251E-522.3150391
CACGCGT1102.926906E-522.27113266
GTCCGCA10750.022.13794913
GAAGCGT10200.021.95921169
TGGCGTG3050.021.84471
AGTCCGC11050.021.53733312
CGGCTAG1003.8853596E-421.0398961
TGTCGGC7400.020.8093230
AAGCGTA11100.020.80931970
CGGCGGC7700.020.4530833
TAGTCCG12000.020.4159911
CGGACGA1901.346416E-820.2765713
GCGGTGA4600.019.8010542
Produced by FastQC (version 0.11.5)