FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3702649
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133570.3607417284220027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG4200.026.7280081
TGTCGGC12800.025.42759930
CGCCCAG2400.024.8486941
CGCGCAG1301.574299E-724.2865071
AAAGGGG40850.023.55955770
CGGCGGC13800.023.33141533
CCGTATC65250.023.11718855
CGGCAAG6800.022.6991521
CGTATCA67050.022.4965956
AAAAGGG78700.022.45655670
CGAGCAG5450.021.8850881
CGAGATC11400.021.8064675
CGCCGTA73550.021.60315953
CGCGCTA1304.263462E-621.5880051
TCGCCGT77050.020.8943752
TCGGCGG15850.020.75539232
GCCGTAT82500.020.61704454
CATTAAA87100.020.5321861
GTCGCCG95350.020.40734351
GAAGCGT17150.020.20214869
Produced by FastQC (version 0.11.5)