FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8374785
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG312790.37349018512117027No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG138550.031.01876470
CGAGATC37600.030.2648335
CCGTATC230350.028.91199355
CGTATCA232750.028.64394256
CGCCGTA260900.027.833953
CCGGATC41100.027.58866355
TCGCCGT262650.027.48855652
GTCGCCG318350.027.39495851
AAAAGGG295900.027.30936470
GCCGTAT280350.027.063854
GGTCGCC353250.026.72927950
CTCGGTG270950.026.41352844
GATCTCG272250.026.2615441
ATCTCGG279950.026.15175842
TCTCGGT270650.026.1193943
TGGTCGC338350.026.11680649
AAGATCG163950.026.0936666
CTAGATC35500.025.5454885
CGGATCA44400.025.53815556
GTATCAT240850.025.52995357
Produced by FastQC (version 0.11.5)