FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8923221
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290520.3255775016667188No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAG6650.025.3199521
TGTCGTG27000.020.2676851
CGGGCAG9700.020.2516121
CGCGAAG12450.020.0047111
CGGCAAG24550.019.1468181
CGGGCTG4650.018.1051271
CGCGTAG3950.017.7427232
TGTCGGC27700.017.30884430
CGCGCAG3650.017.2990781
CGAGCAG15050.017.2479921
GTCCGCA27100.017.17550813
CGGCGGC29050.017.10682733
CGGGAAG88950.016.7210371
AGTCCGC27850.016.71306612
TGCGGTG16550.016.5325531
CGGCCAG5950.016.5076161
CGCGGAT6600.016.476351
TGCGTCG13850.016.462921
TAGTCCG28750.016.19060111
GCGGAGG26300.015.7222122
Produced by FastQC (version 0.11.5)