FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_vk10-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_vk10-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8800707
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG301350.34241567183181987No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG105490.11986536990721314No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC95070.10802541204928194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGTG29250.028.1800561
AAAGGGG102450.025.85921970
CGGGCAG8600.024.4710521
CGCGAAA14400.023.6270241
CGGCGGC34900.023.4650733
TGCGTCG16400.022.4566651
TGTCGGC36200.022.23582330
AAAAGGG228000.021.3819770
CGGCAAG22750.020.9680161
CGCGAAG13450.020.8625561
GTGGGAC39600.020.76774419
GTCGGCG39200.020.71265831
CGAGCAG19000.020.675891
CGCGTAG4450.020.4741232
CGCCAAG19050.020.2533821
TCGGCGG39950.020.1484932
CGCGCAG4550.020.0429571
CGGCAAA65450.019.4534591
CGGGCTG4750.019.1990411
CGGGAAG102800.018.902381
Produced by FastQC (version 0.11.5)