FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10123588
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3012312.9755359463462954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC49150.023.33741655
AAAGGGG47750.022.51204170
CGTATCA52550.022.293256
CGAGATC12200.021.7919315
CGCCGTA56200.021.34534353
CTCGGTG57300.020.9559744
GCCGTAT63250.020.9547954
TGTCGTG8550.020.9094141
TCGCCGT56700.020.84849752
TCTCGGT56350.020.50152643
GATCTCG58600.020.43719941
GTATCAT52850.020.11918857
AAGATCG58250.019.5168656
AGATCTC62750.019.30879840
CCGGATC13050.019.29409655
TCATTAA71250.019.0630160
CAAGATC22650.018.9967295
CGGGAAG18500.018.9481181
CGGGCTA1902.9643888E-718.4494821
TCGCCGG17700.018.37598852
Produced by FastQC (version 0.11.5)