FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7808654
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG122490.1568644224728103Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC89430.11452678015955119Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA88930.1138864649400524Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCTTTCCCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAG87600.11218322645618567TruSeq Adapter, Index 3 (100% over 37bp)
CACTCTTTCCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGG86000.11013421775378958TruSeq Adapter, Index 3 (100% over 38bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC85150.10904568188064166Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT182800.051.9993481
CACTCTT458550.029.7040351
GCCCACA19250.028.3829861
ACTCTTT517700.026.2667712
GGGATTC32150.020.57470133
GGCTATA30550.020.39207653
CTCTTTC700500.019.4122873
GTTGGGA34150.019.16476830
TCTTTCC720700.018.7175984
CTTTCCC715700.018.1977445
AGGCTAT34350.018.13618552
GGTATAC35600.017.3027666
CCCTACA591450.017.1665259
AAGGCTA36950.017.14418451
TAAAGGC37150.017.05188849
TTCCCTA694900.016.9932127
TCCCTAC623100.016.979828
TGTTGGG39450.016.85618429
GCTATAA37600.016.84780754
CGACGAG62200.016.43028616
Produced by FastQC (version 0.11.5)