FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3683367
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT52350.047.166981
GCCCACA17500.033.8231661
CACTCTT130100.022.0481551
GGGATTC14500.021.48201833
GTTGGGA15100.021.09198830
ACTCTTT135850.021.0811422
GGCTATA14700.020.71357253
GGGTGGA9150.020.27252666
TGTTGGG15900.020.25087529
GTGAATG13600.019.0434612
GCCACAC16300.018.9086591
AGGCTAT15800.018.82846552
GCTATAA16500.018.66602354
GGGGGAA17150.018.36671467
TAAAGGC16550.018.18668249
GGGGGGA16400.017.71280366
GGGGAAA19800.017.67615768
AAGGCTA18050.016.67532351
GGGAAAA26700.016.64736469
CTCTTTC172800.016.553083
Produced by FastQC (version 0.11.5)