FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3247834
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAG61280.18867959384623723Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACACTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCA45340.13960073082552865Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC40560.12488322986950687Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40330.1241750655975644No Hit
CACTCTTTCCCTACACGACGCTAGATCGGAAGAGCACACGTCTGAACTCC38630.11894080793538095Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CTCTTTCCCTACACGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTC36860.11349102201651932Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT74200.049.278491
GGTCACG4050.045.80089612
GCCCACA19050.036.4024851
GTCACGA5200.035.67185613
GGAAAAA145050.035.08325270
AGGTCAC5300.034.998811
GGGTGGA43500.034.998866
GTGGAAA90450.034.24426768
GCGTGGA45650.033.8104566
GAAAAAA168100.032.87513770
GGGATTC16150.032.7233433
CGTGGAA47700.032.6508767
TGGAAAA117850.031.86568669
GGTGGAA49100.031.22092467
CGGGGAA8050.030.43373967
GTTGGGA17200.030.3187330
GGGCGTG29550.030.2020164
TCGGCGT33450.030.13349363
GGCGTGA19450.029.51055565
GCGGGGA7750.029.35383266
Produced by FastQC (version 0.11.5)