FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12359200
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGAGAAATGGGTGAATGTTGAGATAATTGTTGGGATTCCATTGTTGATA130670.10572690789047835No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT154250.045.930061
GCCCACA40700.031.8371091
CGCTTGC21150.030.28282423
GGGATTC94950.026.39208433
CACTCTT362750.025.9023721
GCGCTTG25400.025.7669822
GCGTGGA35400.025.7054866
GGCTATA96150.025.2254953
GTTGGGA98800.025.22195230
TAGAGCC27400.024.6593675
GTCCAGC26850.024.37546717
TGTTGGG104300.023.89193529
TAAAGGC101650.023.86060749
AAGGCTA102050.023.83567451
AGGCTAT102250.023.75482252
AGTCCAG28050.023.70698716
TGGGATT108850.023.1183132
GTGAATG90100.023.07370412
GTTGAGA92850.023.03111518
GCTATAA105750.022.80313354
Produced by FastQC (version 0.11.5)