FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6778516
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70190.1035477381775008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT92550.042.7228051
GCGTGGA65850.033.85614866
GGGTGGA56700.032.28289866
CGTGGAA72500.031.23346367
GGAAAAA213650.030.82979270
GTGGAAA134100.030.79690768
GCCCACA24250.030.1841
TCGGCGT52550.029.83727563
CACTCTT201100.029.2228221
GAAAAAA285450.028.76416870
GTCGGCG39400.028.51429762
GGCGTGA31300.028.17799265
GCGTGAA59300.028.15253666
GCACCCA24450.027.6452621
TGGGGGT67800.027.56547263
GGGCGTG40650.027.46527764
GGTGGAA68800.027.21568167
CGTGAAA64400.026.52087467
TGGAAAA189050.026.2699769
CGTCGGC41350.026.23856461
Produced by FastQC (version 0.11.5)