FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6835148
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78180.11437938139744744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAA187600.036.09974370
ACACTCT69250.035.4994131
GGGTGGA48200.033.1111166
CGGGGTT132200.030.4719249
GCCCACA26950.029.1067091
TCGGGGT148500.028.75340848
GTTGGCG61200.028.59397753
GGTTGGC67750.027.37931352
CACTCTT144750.026.9507261
GCGGGGT105300.026.6231957
GGGAAAA189200.026.54524670
TTGGCGG71750.026.3895154
GGTGGAA61050.026.14177967
TGGCGGG122650.025.93894655
TGGGGGT76600.025.8152163
GTGGAAA73000.025.31436368
GGGGGAA164900.024.98111368
GGGGAAA171350.024.77608569
CGCGAGA9800.024.6486633
CGGGGTG126250.024.58941758
Produced by FastQC (version 0.11.5)