FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3539202
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39290.11101372569296694No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT33650.038.0955051
GCGTGGA19800.037.473466
GGGTGGA12750.032.66549366
GGCGTGG16150.031.85644165
CGTGGAA23650.031.81704167
TCGGCGT21750.031.2172763
CGGCGTG21350.031.14642164
GCTTGAA114600.030.23451666
CTGCTTG107550.030.03611464
GGGCGTG10850.029.9989264
CTGCGTG24850.029.71724164
GTGGAAA39900.029.5603468
GCGTGAA33250.029.36736966
TGCTTGA105800.028.91200465
GAAAAAA196550.028.615170
GCCCACA14950.028.5822491
CGGCTTG17450.028.48035264
GGCGTGA14750.028.47355565
CACTCTT72750.028.1644081
CGTGAAA36950.027.37384867
Produced by FastQC (version 0.11.5)