FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402048
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86890.13572219389795265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT61100.042.6469761
GGAAAAA188200.038.6252670
GGTTGGC57000.030.88500452
TTGGCGG56150.030.72922754
GTTGGCG52100.030.69962153
CGGGGTT145500.030.62104249
GGGTGGA45250.030.08746166
GGGAAAA227200.030.05408370
CACTCTT140350.029.9949781
TCGGGGT162400.029.82665648
TGGCGGG117650.028.79638355
CGGAGTC8050.027.3904537
GCGGGGT105100.027.37307757
GCCCACA31950.027.295121
AAAGATC93500.027.1836915
ACGAGTG8350.026.8255138
CGAGTGA8200.026.46259139
GGGGAAA227000.026.39565569
GAGATCG213200.026.1091866
GCACCCA23250.026.0603141
Produced by FastQC (version 0.11.5)