FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4833825
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCACTCTTCTTCTGAAGAGTTAAGCACTCCATTATGCTCATTGGGTTG55060.1139056544248085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT24750.021.2250351
GCCCACA12500.019.8921011
CCTATGC16600.015.60189512
GGCTTCC20200.013.1678837
CCCTACA40600.012.5857929
CGCGGGC3501.0416843E-611.99960468
GGTGCCG30700.011.74228449
CGCGGGG9250.011.72934270
GGACGCG3903.02849E-711.66628366
GCCCGCA29050.011.56588539
TCGCCCG29850.011.37315937
GTGCCGT30850.011.34484550
CCGGTGC30100.011.27869847
CGGTGCC30950.011.19510848
GCCGTAA30750.011.15410352
CATCCGG31050.011.04633444
CCCGCAT30600.010.98003140
ATCCGGT32500.010.76887645
CCTACAC43650.010.66402410
TCCGGTG32200.010.65182346
Produced by FastQC (version 0.11.5)