FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7056092
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98510.13960985769459922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT77600.047.6125031
GGAAAAA262400.039.2402870
GGGTGGA72400.034.90212266
CACTCTT180950.031.2180711
TTGGCGG101350.031.11388854
GTTGGCG84650.031.00898453
GGTTGGC92700.030.8080152
CGGGGTT154900.030.7058649
GCCCACA26450.030.320761
TCGGGGT166100.030.23677648
AAAGATC112700.029.6363985
GGGAAAA288800.029.63022270
GTGGAAA96900.029.61715368
GGTGGAA86100.029.14534867
TGAGATC86850.028.0972585
GCGAGAT32350.027.9219914
GAGATCG262250.027.2209116
GTGAGAT30150.027.1724954
GCGGGGT167100.027.16544257
TGGCGGG187800.027.13432555
Produced by FastQC (version 0.11.5)