FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3872219
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41840.10805173984219385No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT39000.042.9321561
GCCCACA18900.032.8043061
GCGTGGA7250.029.9297368
AGTGGCG6650.028.41981339
GCGGTAG6500.026.92190443
GGCGGTA6700.026.6406342
CACTCTT100000.025.2904551
AACAGGC7350.025.2480813
GGGTGGA10050.024.02880568
GGGCGTG7500.023.79896566
GGGGGGA8700.022.52775468
CGGTAGT8500.022.23432744
TGGCGGT8400.022.0823741
CAGTGGC9100.021.53752338
TGCAGTG9000.020.99908636
ACTCTTT122500.020.69392
CGAGGGC3050.020.65483957
GCGTGAA7800.020.64012768
CCAGAGG9300.020.32169522
TGAGCTG9700.020.20530731
Produced by FastQC (version 0.11.5)