FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945761
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACA14500.039.3752561
ACACTCT24800.035.5920221
CACTCTT66250.022.7345521
AGTGGCG4200.022.49905639
GTGGCGG4300.021.9758240
GGCGTGA3200.021.87408367
TACGCGC1604.9196387E-821.87408322
GCGTGAA6600.020.68095268
GCGGACA2252.1282176E-1020.22137347
GCGGTAG4750.019.15709143
GGCGGTA4850.018.76209842
ACTCTTT80750.018.6449012
GTACGCG1702.1229189E-618.52863521
GCCACAC13050.017.9832461
CCCTACA64150.017.840259
AGGGGGG3153.6379788E-1217.7915271
ATGCCGG6900.017.75287870
CGCTCAG3850.017.27200319
CGACGCG2301.2101009E-716.73842616
CGGTAGT5250.016.66596844
Produced by FastQC (version 0.11.5)