FastQCFastQC Report
Tue 22 Dec 2020
HKTMGAFX2_n01_vk10-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKTMGAFX2_n01_vk10-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2788513
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42280.15162202937551306No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT29800.041.138841
GCCCACA17350.038.357861
AGTGGCG4650.030.10625539
GCGTGAA8550.029.47243768
GCGGTAG4050.029.38147543
GGGTGGA4300.028.48716768
GGCGGTA5150.026.50373542
GGCGTGA4650.026.34297267
GCGTGGA5450.026.32916368
CGTGAAA10700.024.85876369
GGGCGTG4150.024.45679766
CTGCGTG5600.024.3739766
CGGTAGT4800.024.06148344
AGGCTTG5850.023.3423926
GCCACAC16650.023.1408181
TGGCGGT5650.022.91938641
TGAGCTG6000.022.74903931
GTGGGCG5300.022.45188164
CAGAGGT6100.022.37610223
GAGCTGC5800.022.32664332
Produced by FastQC (version 0.11.5)